Gas Chromatography-Mass Spectrometry Analysis of Chemical Compounds in Ethanolic Extracts of Guiera senegalensis and Geigeria alata

Background and Objective: Plants are considered as the primary source of drugs for humans. Plants are rich sources of primary and secondary metabolites. Over the previous decades, plants possessed biologically active metabolites, which have been proven powerful natural medicines. The objective of this research work was to explore the compounds present in the ethanolic extracts of two important Sudanese plant species, namely Guiera senegalensis and Geigeria alata by implicating Gas Chromatography-Mass Spectroscopy (GC/MS) technique. Materials and Methods: The powdered plants samples were extracted with ethanol using a hot extraction technique. The qualitative and quantitative analysis of the samples were carried out by using Gas Chromatography/Mass Spectrometry (GC/MS). Results: The main components of the ethanolic extract of the Guiera senegalensis were 1,2,3-benzenetriol (21%), ethyl alpha-d-glucopyranoside (16.72%), 4H-1-benzopyran-4-one, 2-(3,4-dihydroxy (12%) and quinic acid (11.73%). The main components of the ethanolic extract of Geigeria alata were azuleno[6,5-b]furan-2,6(3H,4H)-dione,3a (26.69%), n-hexadecanoic acid (8.72%) and 1,3-propanediol, 2-(hydroxymethyl)-2- nitro (5.90%). Conclusion: Guiera senegalensis is a rich source of sugars, alcohols and ketones whereas G. alata is a rich source of ketones, fatty acids, alcohols and steroidal compounds.


INTRODUCTION
Plants are considered as the primary source of drugs for humans.Plants are rich sources of primary and secondary metabolites.Over the previous decades, plants possessed biologically active metabolites, which have been proven powerful natural medicines.
Guiera senegalensis L., a plant of the family Combretaceae, commonly known as "Gubeish", has played a very important role in traditional medicine in Africa including West Central Sudan, the leaves can be used as medicine to treat gastrointestinal disorders, cough and topically for wound healing.The leaves contain proanthocyanidins: (-)-epicatechin and (-)-epigallocatechin units.The ethanolic extract contains flavonoids, alkaloids, tannins, phenols, saponins, coumarins and terpenoids [1][2][3] .The studies of chemical composition showed that the main chemical components were beta-carboline alkaloids, harman, tetrahydroharman, guieranone A (a naphthyl butanone) it was exhibited potent antifungal activity against Cladosporium cucumerinum.Ethyl acetate fraction of Guiera senegalensis extract found to exhibit fungicidal property 4 .
Methanolic extract of G. senegalensis extract contain the sugars, phospholipids, phytosterols, guieranone A, porphyrin-containing compounds and phenolics 5 .Alkaloidal extract, beta-carboline from G. senegalensis leaves presented an interesting antiplasmodial activity associated with a low cytotoxicity.
This findings justified to traditional use of the plant to treat malaria 6 .Investigations on G. senegalensis have demonstrated to possess anti-inflammatory, antioxidant, antiasthmatic, anticancer and antimicrobial 1,2 .
Geigeria alata, a plant of the family Asteraceae is commonly known as "Gud-gat".It has been used in West Central Sudan as a drug to treat diabetes, cough, epilepsy and intestinal complaints, antispasmodic, rheumatism and antihypertension [7][8][9] .Geigeria alata aerial parts essential oil contains alpha-longipinene, beta-caryophyllene, alpha-oxobisabolene and caryophyllene oxide 10 .The oil showed moderate in vitro cytotoxicity against some tumor cells 11 .The Trans-3,5-dicaffeoylquinic acid from Geigeria alata roots is slightly toxic and is a potent alpha-glucosidase inhibitor, reduces blood glucose and blood pressure in diabetic hypertension rats and prevents histopathological changes related to diabetes and hypertension 9 .
A sesquiterpene lactone, geigerianolide and flavonoids such as axillarin, quercetin and hispidulin were isolated from G. alata 12 .Acyquinic acids in G. alata exhibit antioxidant and antimicrobial capacity 13 .
Aqueous extract of G. alata after 14 days did not produce any significant change (p<0.05) on haematological parameters tested relative to their respective control group 14 .Analysis of two medicinal plants, Guiera senegalensis and Geigeria alata growing wild under Sudanese environmental conditions is important as there is little or no information on their chemical composition of ethanolic extracts.
In this study, the GC/MS technique was used, which is useful in detecting the compounds in studied plants and concentration distribution.The present investigation was carried out to determine the possible chemical components from ethanolic extracts of candidate plants using gas chromatography/mass spectrometry.Gas Chromatographic-Mass Spectrometry (GC/MS) analysis: The GC was equipped with MS (Shimatzo QP 2010 GC/MS instrument equipped with reference libraries).Packed material for column were 50% phenyl and 50% methyl polysiloscane, column length 30 m, diameter 0.025 mm, the flow rate of helium as carrying gas was 1 mL/min, the temperature of program consisted of 60-270°C , at rate of 4°C/min.The MS were taken at ionization voltage 70 EV.Library Search was carried out using Wiley GC/MS library.Interpretation on mass spectrum GC/MS was conducted using the database of National Institute Standard and Technology (NIST).The spectrum of the unknown component was compared with the spectrum of the known components stored in the NIST library.The retention time (TR), name of compound and area (%) of the components of the test materials were ascertained.
The biological activities of some components, n-hexadecanoic acid (palmitic acid) possesses some activity like antioxidant, hypercholesterol, nematicidal and pesticide 15 .Gamma-sitosterol was previously reported to possess antihyperglycemic activity by increasing insulin secretion in response to glucose 16 .Linoleic acid was found to possess antibacterial activity against Bacillus megaterium at MICs of 0.2 and 0.05 mM 17 .
Recent investigations with phytol demonstrated anxiolytic, metabolism-modulating, cytotoxic, antioxidant, autophagy and apoptosis-inducing, antinociceptive, anti-inflammatory, immune-modulating and antimicrobial effects 18 .The beta-sitosterol was found to possess anti-inflammatory, anticancer, antioxidant, hepatoprotective, cardioactive and antidiabetic 19 .The 1,2,3-benzenetriol (Pyrogallol) has the ability to perform antibacterial and antioxidant activities 20 .The ethyl-alpha-d-glucopyranoside, skin moisturing agent 21 .The consumption of a food or beverage enriched with sucrose has been associated with improve mental alertness, memory, reaction time, as well as a reduction in the feeling of fatigue 22 .Oleic acid was found to have antibacterial activity, particularly in inhibiting the growth of several Gram-positive bacterial species 23

CONCLUSION
The ethanolic extracts from Guiera senegalensis and Geigeria alata has revealed a wealth of diverse bioactive compounds, identified and quantified through Gas Chromatography-Mass Spectrometry (GC/MS).Guiera senegalensis displayed a significant presence of sugars, alcohols and ketones, suggesting its potential for therapeutic applications.Conversely, Geigeria alata exhibited a distinct chemical profile rich in ketones, fatty acids, alcohols and steroidal compounds, hinting at its potential for various physiological activities.These findings underscore the importance of botanical exploration in the search for new therapeutic agents and highlight the chemodiversity inherent in Sudanese plant species, thus offering valuable insights into the potential utilization of these botanical resources in drug discovery and development efforts.

SIGNIFICANCE STATEMENT
This study aimed to explore the compounds present in the ethanolic extracts of two important Sudanese plant species namely, Guiera senegalensis and Geigeria alata by implicating Gas Chromatography-Mass Spectroscopy (GC/MS) technique.The present study revealed that the ketonic, alcoholic, fatty acids and sugar derivatives compounds in studied two plants may be explored for manufacturing industrial products.
area: This study was designed and carried out in the Department of Botany, Faculty of Science and Technology, Omdurman Islamic University, Omdurman, Sudan between November, 2016 and March, 2017.Plant material: Plants samples, Guiera senegalensis and Geigeria alata were purchased from Omdurman Local Market in November, 2016.The voucher specimens were at Herbarium (Ref No. 430 and 512, respectively) of Department of Botany, Faculty of Science and Technology, Omdurman Islamic University.Preparation of ethanolic extracts: The powdered samples were extracted with ethanol using a hot extraction technique.The powder was extracted separately with 50 mL ethanol by gentle reflux for 1 hr.After extraction, it was filtered and then the removal of solvent was done by a rotary evaporator.Then the (15 mL) was kept in a glass container.https://doi.org/10.3923/ajbs.2024.360.365| Page 361

Table 2 :
Chemical constituents of Geigeria alata aerial parts ethanolic extract