Detection of mecA and PVL-Resistant Genes in Staphylococcus aureus from Clinical Samples in Bayelsa State, Nigeria

Background and Objectives: Methicillin-resistant bacteria Staphylococcus aureus (MRSA) is known to cause serious infections with a wide range of severity and antibiotic susceptibility. The purpose of this study was to identify mecA and PVL genes in MRSA. Materials and Methods: Using standard bacteriological techniques, MRSA was isolated from clinical samples. The boiling method was used to extract genomic DNA. In an AB1970 applied biosystem thermal cycler, mecA and PVL were amplified. A total of 250 chemical specimens were collected, with urine samples, wound samples and endocervical swabs. Results: High vaginal swabs accounting for 136 (54.4%), 49 (19.6%), 4 (16%) and 25 (10), respectively. Of the 25 MRSA isolates, 11 (44%) came from urine, 10 (40%) from the wound, 4 (16%) from the higher vaginal swab and two from the endocervical swab. The MRSA was found in the highest concentrations in urine. The susceptibility pattern of MRSA isolates revealed that the organisms were highly resistant to Ampiclox 25 (100%), Amoxicillin 21 (84%), Streptomycin 19, (76%) and Gentamycin 5 (20%). None of the 25 MRSA isolates contained mecA or PVL genes. Conclusion: The findings highlight the importance of continuously monitoring the methicillin resistance pattern of MRSA isolates, as well as the need for stringent infection control measures to prevent transmission and spread.