Prevalence and Antimicrobial Susceptibility Testing and Analysis of Microbes Isolated from Different Clinical Samples

Background and Objective: In developing countries, the most crowded buildings in each city are hospitals and clinics due to the emergence of multidrug-resistant microbes. Therefore, the present study was conducted to find the resistance pattern of these microbes. Materials and Methods: A total of 2209 samples of blood, pus, tissue and urine were collected and cultured on their respective media plates. The culture-positive plates were identified through staining and biochemical methods. Antimicrobial susceptibility testing and statistical analysis, i.e., One-way ANOVA and post-hoc tests, were performed to check the resistance pattern and their significance. Results: Among cultured-positive samples, the most commonly isolated gram-positive cocci were Staphylococcus aureus and Enterococcus spp., which were resistant to Penicillin and Erythromycin and sensitive to Vancomycin and gram-negative rods were Escherichia coli and Klebsiella pneumoniae that were resistant to Ciprofloxacin and Ceftriaxone and sensitive to Polymyxin-B and Tigecycline. This study also witnessed the emergence of Serratia spp., as a multi-drug resistant bug and Proteus spp., as a secondary pathogen in skin and soft tissue infections. Conclusion: The antibiotics that are found to be highly resistant in this study should be stopped and microbial examination should be made compulsory before going for any treatment.