Psychrophilic Amylase and Protease Production Using Aspergillus oryzae CP3

Background and Objective: Majority of industrial enzymes are produced by fungi. Psychrophilic hydrolytic enzymes are required for cold water detergents, biosensors food additives etc. The aim of present experiments is to produce Psychrophilic Amylase and Protease enzymes by SSF and SMF to get high production yields of both enzymes. Materials And Methods: Aspergillus oryzae CP3 was isolated from food preserved in refrigerators and found to produce amylase and protease. The strain was used for amylase and protease production by SSF and SMF. The SSF was conducted with rice and wheat brawns supplemented with nutrition broth. Results: More amylase is produced (7800 IU/gds) in SSF where as more protease is produced (500 IU/gds) in SMF. Further impact of temperature calcium and chloride ions, specific activity of enzymes was tested and temperature 25̊C, pH 8.5, Ca²⁺ (100 Mm), Cl‾ (300 mm) were found for the high specific activity of both enzymes. Hence, these enzymes are highly suitable for cold detergents application. Conclusion: The study concludes that the isolated Aspergillus oryzae can be a potent source for amylase and protease production. Amylase and protease produced can be used in cold detergents.